The positive role of Bacillus exopolysaccharides (EPS) on the enhancement of induced systemic resistance (ISR) in tomato – F. oxysporum f.sp. lycopersici pathosystem was studied under pot culture condition by employing the Bacillus EPS wild and EPS negative mutant strains of Bacillus together with challenge inoculation of F. oxysporum f.sp. lycopersici. It was observed that the application of Bacillus EPS wild and EPS negative mutant altered the biochemical and physiological characteristics viz., level of reducing and non-reducing sugars, total phenol content and defense enzyme activities, such as, peroxidase (PO), polyphenol oxidase (PPO), of tomato plant to a higher level when compared to control.