Two efficient strains (AOB-6 and NOB-4) of ammonia oxidizing bacteria and nitrite oxidizing bacteria have been isolated in pure culture by liquid enrichment techniques from shrimp culture pond bottom soil. Both were identified as gram negative, rod shaped and similar to the genera Nitrosomonas (AOB-6 KT231978) and Nitrobacter (NOB-4 KT231980) by 16s RNA sequencing. The both strains of AOB-6 and NOB-4 were studied on bioconversion of ammonium to nitrite and nitrite to nitrate at different pH and temperature respectively. The strain AOB-6 has been recorded highest ammonium oxidation to nitrite pH 8 and Temperature 30˚C.

Water is one of the five elements that are ‘Akash, Vayu, Teja, Jala, & Pruthvi’, by which body is constructed, according to vaidic thoughts. Scientifically water is gifted with unusual physico-chemical properties which are responsible for its specificity. Water is indeed a most essential commodity for human consumption of without it no life can exist. Water is the most widely distributed resource of the earth and unlike other mineral resources; it gets its annual replenishment from the meteoric precipitation.